anti hcn2 (Proteintech)
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Proteintech
anti hcn2
Anti Hcn2, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+hcn2/HCN2+Antibody/pm41258586-99-9-11
Average 93 stars, based on 14 article reviews
Anti Hcn2, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+hcn2/HCN2+Antibody/pm41258586-99-9-11
Average 93 stars, based on 14 article reviews
anti hcn2 - by Bioz Stars,
2026-10
93/100 stars
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Incubation:Article Title: HCN channels in the lateral habenula regulate pain and comorbid depressive‐like behaviors in mice Article Snippet: All sections were rinsed using PBS and incubated in a blocking solution containing 0.3% Trion X‐100 (X‐100, Sigma, USA), 1% bovine serum albumin (A4503, Sigma, USA), and 1% normal donkey serum (ab7475, Sigma, USA). .. Subsequently, the sections were incubated with primary antibodies including anti‐HCN1 (55222‐1‐AP, 1:200, rabbit, Proteintech, USA), Article Title: Thalamocortical Circuit Controls Neuropathic Pain via Up-regulation of HCN2 in the Ventral Posterolateral Thalamus Article Snippet: .. Subsequently, the membrane was blocked at room temperature for 1 h and incubated with rabbit polyclonal anti-HCN1 (1:1000; Proteintech, IL, USA) and Article Title: HCN2 contributes to oxaliplatin-induced neuropathic pain through activation of the CaMKII/CREB cascade in spinal neurons Article Snippet: .. The membranes were placed in the block buffer at room temperature for 1 h and incubated with one of the following primary antibodies: Article Title: HCN2 contributes to oxaliplatin-induced neuropathic pain by inducing spinal long-term potentiation via activation of NMDA receptor-mediated CaMKII signaling. Article Snippet: Our previous findings indicate that HCN2 contributes to oxaliplatin-induced neuropathic pain, but the mechanisms underlying the development of neuropathic pain are still unclear.. Here, we found that the rat HCN2 levels significantly increased after high-frequency stimulation–induced long-term potentiation (LTP).. Spinal local application of ZD7288 (a cyclic-nucleotide-gated-channel–specific inhibitor) prevented LTP induction after intraperitoneal injection of oxaliplatin. Cell-Signaling:Article Title: HCN channels in the lateral habenula regulate pain and comorbid depressive‐like behaviors in mice Article Snippet: All sections were rinsed using PBS and incubated in a blocking solution containing 0.3% Trion X‐100 (X‐100, Sigma, USA), 1% bovine serum albumin (A4503, Sigma, USA), and 1% normal donkey serum (ab7475, Sigma, USA). .. Subsequently, the sections were incubated with primary antibodies including anti‐HCN1 (55222‐1‐AP, 1:200, rabbit, Proteintech, USA), Blocking Assay:Article Title: HCN-Channel-Dependent Hyperexcitability of the Layer V Pyramidal Neurons in IL-mPFC Contributes to Fentanyl-Induced Hyperalgesia in Male Rats. Article Snippet: Then, brains were cryoprotected in 20% sucrose for 24 h at 4 °C followed by 30% sucrose for 24 h. Brain free-floating Sects. (30 μm) cut on vibration microtome (LEICA VT1200S) in chilled 0.1 M PBS were prepared for immunohistochemical staining. .. QuickBlockTM Blocking Buffer for Immunol Staining (Beyotime, Wuhan, China) was used to block the brain slices in a 24-well Tissue Culture Plate (Biofil, Guangzhou, China) for 10 min at room temperature, then incubating the Sects. (12–24 h at 4 °C) with the primary antibodies: anti c-Fos (1:300, rabbit, CST technology), anti-HCN1 (1:100, rabbit, Abclonal), Article Title: HCN2 contributes to oxaliplatin-induced neuropathic pain through activation of the CaMKII/CREB cascade in spinal neurons Article Snippet: .. The membranes were placed in the block buffer at room temperature for 1 h and incubated with one of the following primary antibodies: Staining:Article Title: HCN-Channel-Dependent Hyperexcitability of the Layer V Pyramidal Neurons in IL-mPFC Contributes to Fentanyl-Induced Hyperalgesia in Male Rats. Article Snippet: Then, brains were cryoprotected in 20% sucrose for 24 h at 4 °C followed by 30% sucrose for 24 h. Brain free-floating Sects. (30 μm) cut on vibration microtome (LEICA VT1200S) in chilled 0.1 M PBS were prepared for immunohistochemical staining. .. QuickBlockTM Blocking Buffer for Immunol Staining (Beyotime, Wuhan, China) was used to block the brain slices in a 24-well Tissue Culture Plate (Biofil, Guangzhou, China) for 10 min at room temperature, then incubating the Sects. (12–24 h at 4 °C) with the primary antibodies: anti c-Fos (1:300, rabbit, CST technology), anti-HCN1 (1:100, rabbit, Abclonal), Membrane:Article Title: Thalamocortical Circuit Controls Neuropathic Pain via Up-regulation of HCN2 in the Ventral Posterolateral Thalamus Article Snippet: .. Subsequently, the membrane was blocked at room temperature for 1 h and incubated with rabbit polyclonal anti-HCN1 (1:1000; Proteintech, IL, USA) and Article Title: HCN2 contributes to oxaliplatin-induced neuropathic pain by inducing spinal long-term potentiation via activation of NMDA receptor-mediated CaMKII signaling. Article Snippet: Our previous findings indicate that HCN2 contributes to oxaliplatin-induced neuropathic pain, but the mechanisms underlying the development of neuropathic pain are still unclear.. Here, we found that the rat HCN2 levels significantly increased after high-frequency stimulation–induced long-term potentiation (LTP).. Spinal local application of ZD7288 (a cyclic-nucleotide-gated-channel–specific inhibitor) prevented LTP induction after intraperitoneal injection of oxaliplatin. Software:Article Title: HCN2 contributes to oxaliplatin-induced neuropathic pain through activation of the CaMKII/CREB cascade in spinal neurons Article Snippet: .. The membranes were placed in the block buffer at room temperature for 1 h and incubated with one of the following primary antibodies: Transfection:Article Title: Non-CpG methylation by DNMT3B facilitates REST binding and gene silencing in developing mouse hearts Article Snippet: .. Primary embryonic and neonatal ventricular cardiomyocytes were seeded onto Matrigel-coated Nunc Thermanox and poly-L-lysine/laminin-coated glass coverslips, transfected with 100 nM siRNA for Dnmt3a, Dnmt3b or Rest by the Lipofectamine LTX and PLUS Reagents for 48 h, and then exposed to |
